The 34-year-old midfielder stepped away from international duty after Euro 2020 when Die Mannschaft were knocked out by England in the quarter-finals.
However, Kroos announced at the end of last month that he is «in the mood» to come out of retirement and represent Euro 2024 host nation Germany this summer.
The 2014 World Cup winner, who has earned 106 international caps, has now officially been included in Nagelsmann's latest squad ahead of friendlies away against France on March 23 and at home to the Netherlands on March 26.
Meanwhile, Bayern Munich midfielder Aleksandar Pavlovic is one of six players along with Maximilian Beier, Deniz Undav, Waldemar Anton, Maximilian Mittelstadt and Jan-Niklas Beste to receive their first senior call-up to the Germany squad.
Munich-born Pavlovic is of Serbian decent and although the Serbian FA sent a delegation to meet the midfielder and his family earlier this month, the 21-year-old has opted to represent Germany.
Another notable inclusion in Nagelsmann's squad is Bayern goalkeeper Manuel Neuer, who was omitted from the previous Germany setup at the end of last year to focus on club duties.
The 37-year-old is expected to regain the number-one jersey from Marc-Andre ter Stegen, while Bernd Leno and uncapped Oliver Baumann are two more shot-stoppers at Nagelsmann's disposal.
In defence Chris Fuhrich and Robin Koch have both returned to the senior squad, the latter receiving his first call-up for nearly three years.
Barcelona's Ilkay Gundogan will continue as Germany captain and is one of only three midfielders out of the eight called up, along with Kroos and Jama
Read on sportsmole.co.uk