The Three Lions captain picked up an ankle injury during Bayern Munich's most recent Bundesliga clash with SV Darmstadt 98, although he still linked up with the squad at St George's Park.
Little is known about the severity of Kane's issue, although he was one of a handful of players to miss team training on Wednesday, instead undergoing individual work in the gym.
The 30-year-old's problem has also poured doubt on his availability for Bayern's Champions League quarter-final with Arsenal next month, and Southgate's latest admission will not be music to the ears of Thomas Tuchel.
Speaking to the media in his pre-game press conference on Friday evening, Southgate announced that Kane would not be fit to take on Brazil, and nor would Chelsea's Cole Palmer and Ajax's Jordan Henderson.
Both men also sat out team training this week alongside Bukayo Saka, who withdrew from the squad on Thursday, but Southgate is keeping his fingers crossed that Palmer and Henderson can make themselves available for the meeting with Belgium next Tuesday.
«Tomorrow, no Harry Kane, no Jordan Henderson, no Cole Palmer,» Southgate told reporters. «I would say Cole and Hendo have a better chance of Belgium than Harry, he'd be extremely doubtful for that.»
With England's all-time record goalscorer unavailable, Southgate must choose between Aston Villa's Ollie Watkins and Brentford's Ivan Toney to lead the line, while Jarrod Bowen or Phil Foden could benefit from Saka and Palmer's absence to start on the right.
Alternatively, Foden could vie for Henderson's spot in midfield, although Conor Gallagher and the
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